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tissue culture cells ncm460  (ATCC)


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    ATCC tissue culture cells ncm460
    The cytotoxic activities of the peptides were measured in (A) <t>NCM460</t> non-cancerous, (B) SW480, and (C) Caco2 cancer cell lines using MTT assay. Triton X-100 (1%) was utilized as a positive control for cytotoxicity. The data represent the mean ± SD of a representative assay (n = 3). The dotted line corresponds to the IC 50 value or 50% cytotoxicity, which defines the pharmacological potency of the molecule.
    Tissue Culture Cells Ncm460, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue+culture+cells+ncm460/L+Cells/pmc12507217-65-0-10
    Average 99 stars, based on 4442 article reviews
    tissue culture cells ncm460 - by Bioz Stars, 2026-09
    99/100 stars

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    1) Product Images from "Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides"

    Article Title: Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides

    Journal: PLOS One

    doi: 10.1371/journal.pone.0334029

    The cytotoxic activities of the peptides were measured in (A) NCM460 non-cancerous, (B) SW480, and (C) Caco2 cancer cell lines using MTT assay. Triton X-100 (1%) was utilized as a positive control for cytotoxicity. The data represent the mean ± SD of a representative assay (n = 3). The dotted line corresponds to the IC 50 value or 50% cytotoxicity, which defines the pharmacological potency of the molecule.
    Figure Legend Snippet: The cytotoxic activities of the peptides were measured in (A) NCM460 non-cancerous, (B) SW480, and (C) Caco2 cancer cell lines using MTT assay. Triton X-100 (1%) was utilized as a positive control for cytotoxicity. The data represent the mean ± SD of a representative assay (n = 3). The dotted line corresponds to the IC 50 value or 50% cytotoxicity, which defines the pharmacological potency of the molecule.

    Techniques Used: MTT Assay, Positive Control

    Related Articles

    Cell Culture:

    Article Title: Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides.
    Article Snippet: .. Tissue culture cells NCM460 (normal human colon mucosal epithelial cells) ATCC CVCL-0460, SW480 ATCC CCL228, and Caco2 (colorectal adenocarcinoma cells) ATCC® HTB-37TM were obtained from ATCC (Manassas, VA, USA) and cultured under standard conditions using Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Sigma-Aldrich Co., St. Louis, MO, USA) in a humidified incubator with an atmosphere of 95% air and 5% CO 2 at 37° C. DMEM was supplemented with 10% heat-inactivated fetal bovine serum (FBS, Invitrogen Ltd, Paisley, UK), 2 mM L-glutamine, 1 mM sodium pyruvate, sodium bicarbonate, 1% non-essential amino acids, 100 units/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich Co., St. Louis, MO, USA). .. The peptides Ib-M1, Ib-M2, and Ib-M6 were obtained from GenScript® (Piscataway, NJ, USA) using solid-phase 9-fluorenyl methoxycarbonyl (Fmoc) chemistry with C-terminal amidation and the free N-terminus.

    Article Title: Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides
    Article Snippet: .. Tissue culture cells NCM460 (normal human colon mucosal epithelial cells) ATCC CVCL-0460, SW480 ATCC CCL-228, and Caco2 (colorectal adenocarcinoma cells) ATCC® HTB-37TM were obtained from ATCC (Manassas, VA, USA) and cultured under standard conditions using Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Sigma-Aldrich Co., St. Louis, MO, USA) in a humidified incubator with an atmosphere of 95% air and 5% CO 2 at 37° C. DMEM was supplemented with 10% heat-inactivated fetal bovine serum (FBS, Invitrogen Ltd, Paisley, UK), 2 mM L-glutamine, 1 mM sodium pyruvate, sodium bicarbonate, 1% non-essential amino acids, 100 units/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich Co., St. Louis, MO, USA). .. The peptides Ib-M1, Ib-M2, and Ib-M6 were obtained from GenScript® (Piscataway, NJ, USA) using solid-phase 9-fluorenyl methoxycarbonyl (Fmoc) chemistry with C-terminal amidation and the free N-terminus.

    Modification:

    Article Title: Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides.
    Article Snippet: .. Tissue culture cells NCM460 (normal human colon mucosal epithelial cells) ATCC CVCL-0460, SW480 ATCC CCL228, and Caco2 (colorectal adenocarcinoma cells) ATCC® HTB-37TM were obtained from ATCC (Manassas, VA, USA) and cultured under standard conditions using Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Sigma-Aldrich Co., St. Louis, MO, USA) in a humidified incubator with an atmosphere of 95% air and 5% CO 2 at 37° C. DMEM was supplemented with 10% heat-inactivated fetal bovine serum (FBS, Invitrogen Ltd, Paisley, UK), 2 mM L-glutamine, 1 mM sodium pyruvate, sodium bicarbonate, 1% non-essential amino acids, 100 units/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich Co., St. Louis, MO, USA). .. The peptides Ib-M1, Ib-M2, and Ib-M6 were obtained from GenScript® (Piscataway, NJ, USA) using solid-phase 9-fluorenyl methoxycarbonyl (Fmoc) chemistry with C-terminal amidation and the free N-terminus.

    Article Title: Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides
    Article Snippet: .. Tissue culture cells NCM460 (normal human colon mucosal epithelial cells) ATCC CVCL-0460, SW480 ATCC CCL-228, and Caco2 (colorectal adenocarcinoma cells) ATCC® HTB-37TM were obtained from ATCC (Manassas, VA, USA) and cultured under standard conditions using Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Sigma-Aldrich Co., St. Louis, MO, USA) in a humidified incubator with an atmosphere of 95% air and 5% CO 2 at 37° C. DMEM was supplemented with 10% heat-inactivated fetal bovine serum (FBS, Invitrogen Ltd, Paisley, UK), 2 mM L-glutamine, 1 mM sodium pyruvate, sodium bicarbonate, 1% non-essential amino acids, 100 units/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich Co., St. Louis, MO, USA). .. The peptides Ib-M1, Ib-M2, and Ib-M6 were obtained from GenScript® (Piscataway, NJ, USA) using solid-phase 9-fluorenyl methoxycarbonyl (Fmoc) chemistry with C-terminal amidation and the free N-terminus.



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    tissue culture cells ncm460  (ATCC)
    99
    ATCC tissue culture cells ncm460
    The cytotoxic activities of the peptides were measured in (A) <t>NCM460</t> non-cancerous, (B) SW480, and (C) Caco2 cancer cell lines using MTT assay. Triton X-100 (1%) was utilized as a positive control for cytotoxicity. The data represent the mean ± SD of a representative assay (n = 3). The dotted line corresponds to the IC 50 value or 50% cytotoxicity, which defines the pharmacological potency of the molecule.
    Tissue Culture Cells Ncm460, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue+culture+cells+ncm460/L+Cells/pmc12507217-65-0-10
    Average 99 stars, based on 1 article reviews
    tissue culture cells ncm460 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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    The cytotoxic activities of the peptides were measured in (A) NCM460 non-cancerous, (B) SW480, and (C) Caco2 cancer cell lines using MTT assay. Triton X-100 (1%) was utilized as a positive control for cytotoxicity. The data represent the mean ± SD of a representative assay (n = 3). The dotted line corresponds to the IC 50 value or 50% cytotoxicity, which defines the pharmacological potency of the molecule.

    Journal: PLOS One

    Article Title: Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides

    doi: 10.1371/journal.pone.0334029

    Figure Lengend Snippet: The cytotoxic activities of the peptides were measured in (A) NCM460 non-cancerous, (B) SW480, and (C) Caco2 cancer cell lines using MTT assay. Triton X-100 (1%) was utilized as a positive control for cytotoxicity. The data represent the mean ± SD of a representative assay (n = 3). The dotted line corresponds to the IC 50 value or 50% cytotoxicity, which defines the pharmacological potency of the molecule.

    Article Snippet: Tissue culture cells NCM460 (normal human colon mucosal epithelial cells) ATCC CVCL-0460, SW480 ATCC CCL-228, and Caco2 (colorectal adenocarcinoma cells) ATCC® HTB-37TM were obtained from ATCC (Manassas, VA, USA) and cultured under standard conditions using Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Sigma-Aldrich Co., St. Louis, MO, USA) in a humidified incubator with an atmosphere of 95% air and 5% CO 2 at 37° C. DMEM was supplemented with 10% heat-inactivated fetal bovine serum (FBS, Invitrogen Ltd, Paisley, UK), 2 mM L-glutamine, 1 mM sodium pyruvate, sodium bicarbonate, 1% non-essential amino acids, 100 units/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich Co., St. Louis, MO, USA).

    Techniques: MTT Assay, Positive Control